Total Testosterone Test: Normal Ranges, Results, and Next Steps
Testosterone is a hormone typically associated with the development and maintenance of male characteristics. In men, it plays multiple roles, such as the development of male sex organs, a deeper voice, facial hair, muscle and bone growth, and sex drive. However, this androgen impacts overall physical health and emotional wellbeing in men and women.
Kristina Cadwell
January 20, 2026
·
6 min read
Imbalanced testosterone levels can provoke or worsen many health conditions. A total testosterone test is a valuable diagnostic tool for discovering the underlying cause behind these conditions.
This article explains what total testosterone tests help determine and why they may be significant for you.
What Is a Total Testosterone Test?
A total testosterone test measures blood hormone levels. Most circulating testosterone is bound to proteins, while 0.5-3% remains unbound (free) and bioavailable.
A total testosterone test measures three testosterone types:
Free or unattached testosterone – A biologically active form that is not bound to proteins and is more readily available. It impacts bodily functions such as libido, muscle mass, and bone health.
Albumin-bound testosterone – Bound to albumin, a blood protein, this type is also bioavailable, though less so than free testosterone.
Testosterone bound to SHBG (sex hormone-binding globulin) – This type is not bioavailable but serves as a reservoir that can be activated when needed.
Other tests include a free testosterone test (which measures only the active form) and a bioavailable testosterone test (which measures free testosterone and the albumin-bound type). These tests are less common but may be help diagnose certain medical conditions.
This test helps determine testosterone levels in adult men and women. It also helps to diagnose early or delayed puberty in children, teenagers, and adolescents.
Both suboptimal and excessive testosterone production can cause or worsen many medical conditions. Your primary healthcare provider may recommend this test if any of the following symptoms apply to you.
Men – Testosterone Levels
High
Low
Acne Insomnia High blood pressure Excessive facial and body hair Infertility Aggression Prostate enlargement Headaches Increased appetite Unexplained weight gain
A total testosterone test is a key indicator of overall testosterone levels in the body. It measures bound and unbound (free) testosterone in the bloodstream and helps to determine the link between abnormal testosterone levels and one’s health condition.
The test is helpful in early diagnosis as it can indicate the following:
The presence of a hormonal imbalance that increases the risk of conditions such as hypogonadism, endocrine disorders, or tumors.
The cause of symptoms such as weight oscillations, mood swings, low energy, and other medical conditions.
The causes of delayed or early puberty in teenagers and adolescents.
Whether the patient’s testosterone decline is due to aging or pathological reasons.
Androgen levels in patients with a history of testosterone-related disorders or abnormal testosterone levels in the past.
The health status of individuals taking medication or undergoing therapies that may affect their testosterone levels (e.g., antiandrogens, glucocorticoids, chemotherapy).
Does a Total Testosterone Test Require Special Preparation?
Testing is typically performed between 7 am and 10 am, when testosterone levels are at their highest. There is no need to prepare for testing unless your medical provider tells you otherwise. Some doctors recommend fasting, during which patients avoid eating and drinking anything but water for several hours before taking the test. You should avoid strenuous physical activities, drinking alcohol, and smoking for 24 hours before testing to avoid hormonal disruption.
Ask your medical provider for specific instructions and inform them about all the supplements, herbs, and medication you are currently taking, including both prescription and over-the-counter drugs.
What Happens During a Total Testosterone Test?
The doctor or a nurse will place an elastic band called a tourniquet around your upper arm and clean the injection site with an antiseptic to prevent infection. They will draw blood from a vein inside your elbow using a thin, sterile needle that transfers the sample into a small vial. The doctor will remove the tourniquet and cover the site with a cotton ball or a bandage to stop bleeding.
The entire process lasts less than a minute. You might feel a slight pinch or a sting as the needle is inserted and removed.
The Results of a Total Testosterone Test
Test results vary depending on your age, gender, and health conditions. To obtain accurate results, your doctor may recommend retaking the test on different days or at separate times of the day.
According to MedlinePlus, an optimal range given in nanograms per deciliter (ng/dL) is as follows:
300 to 1000 ng/dL for men
15 to 70 ng/dL for women
Patients usually receive the results via mail or an online health portal within several days. Optimal value ranges may vary slightly among different laboratories, and each laboratory has a corresponding reference range for a healthy person depending on age and gender. Your doctor will tell you whether your results are within a healthy range in your specific case.
A testosterone test alone does not diagnose any health condition, but it may indicate an underlying issue. You should schedule a follow-up visit with your doctor to discuss the results and next steps. You might need to take additional blood tests to check other hormone levels.
Does Anything Affect the Results of a Total Testosterone Test?
The results can be affected by any of the following:
Medication such as antifungal medicines (e.g., ketoconazole) and hormonal drugs.
Foods with phytoestrogen such as flax seeds, dried fruit, soybeans, tofu, sesame seeds, garlic, and tempeh.
Performing the test later in the day can indicate falsely lower testosterone levels.
Is There Any Risk of Taking a Total Testosterone Test?
Potential risks and side effects are minimal and temporary. They include:
Bleeding
Infection
Bruising or hematoma
Lightheadedness
Soreness at the injection site
Can a Total Testosterone Test Be Taken at Home?
You can check your testosterone levels with an at-home test kit that allows you to collect a blood or saliva sample and send it to a lab for testing. These kits are available without a prescription.
While at-home tests offer convenience and can be useful for initial screening, it is important to note that a physician-ordered blood test is the gold standard for accuracy and reliability.
Total Testosterone Test Price
The total testosterone test cost range in the United States is $17 to $149. The pricing varies depending on the clinic’s location, expertise, and your medical insurance policy.
Some clinics include a consultation and a follow-up visit in the price. Before scheduling an appointment, consult your healthcare provider about additional services and consultation fees.
Conclusion
Testosterone levels decline with age and vary among men and women. A total testosterone test is the most common and comprehensive test used for diagnostic and monitoring purposes. It helps to determine whether your current health concern is linked to this androgen and is the first step in resolving your health issues.
Our friendly Vibrant Skin Bar staff can help you learn more about testosterone and other hormones.
PA Kristina Cadwell graduated from Arizona State University with a Bachelor of Science degree in Biology. She then completed the Physician Assistant program at A.T. Still University graduating with a Masters of Medical Science degree. Kristina has been working in the Aesthetic Medical field for over 15 years. She is certified in neurotoxin cosmetic injection, dermal fillers, laser therapy, chemical peels, and ultrasound. She is known for producing natural results and has extensive knowledge in nutrition, hormone balancing, microbiome health, diet and exercise. Her latest endeavor is the Vibrant Skincare Line, a microbiome-friendly line of skincare that works with your body. Her passion and the reason she does everything she does? Helping you achieve vibrant skin from the inside out.